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The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast https://augustdpzjp.snack-blog.com/26475435/considerations-to-know-about-delequamine

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